Heleneide Resende de Souza Nazareth Laboratory

The Heleneide Resende de Souza Nazareth Laboratory is a multi-user facility affiliated with the Genetics Division. It is equipped with infrastructure suitable for a wide range of molecular experiments, including in vitro cell culture studies.

The laboratory features automated, high-precision platforms for nucleic acid extraction, quantification, analysis, and amplification. Key equipment includes spectrophotometry and fluorometry instruments (NanoDrop™, Qubit®) and nucleic acid integrity analysis systems (Bioanalyzer, Agilent). For gene amplification and analysis, the laboratory is equipped with multiple conventional thermocyclers and medium- to high-throughput real-time PCR systems (Applied Biosystems and Thermo Fisher Scientific), including high-capacity instruments for large-scale quantitative analyses.

The infrastructure also includes capillary sequencing platforms (SeqStudio™), as well as comprehensive cytogenetics and imaging systems, including optical, fluorescence, and inverted microscopes, integrated with specialized cytogenetic analysis software.

To support experimental activities, the laboratory maintains extensive sample storage and preservation infrastructure, including ultra-low temperature freezers (−80 °C), as well as multiple refrigerated and non-refrigerated centrifuges. Cell culture and molecular biology workflows are supported by dedicated laminar flow hoods, incubators, shaking incubators, and gel documentation systems.

Coordinator: Alexandre Ferro Aissa, PhD (aissa@unifesp.br)

Deputy Coordinator: Isabel Cristina Céspedes, PhD